---
title: "Day 1"
canonical: "https://help.biobam.com/space/BTP/3308912641/Day%201"
format: markdown
---
Practical Session 1 - 1:15h  (**Marta + **Priyansh)

DataSet: Use the same datasets for clustering and trajectory analysis. 

1. Healthy Bone Marrow: FASTQ : Cryopreserved bone marrow stem/**progenitor CD34+** cells from 3 **healthy** donors. 10x dataset. 3 different samples
2. Full-length dataset (Smart-Seq or SMARTer)
3. ¿BD Rhapsody or 10x 5’?


Do the count table with a subset of the FASTQ files. Then provide the count table of the full dataset to continue with the downstream analyses.


<u>Data delivery </u>→ Stefan will try this. If it does not work, just put the data link in Confluence.

Data put data in OmicsBox.  Try at Biobam

Distribute the key to participants

Create and distribute a small how-to-get to your data.


Marta designs and tests the whole exercise.


Steps: 

- Intro to OmicsBox
- Intro to Datasets
- Check Data, QC, Experimental Design, Metadata?
- Run Read Quantification (StarSolo)
- Review Results and Interpretation

Practical Session 2 - 45 min  (**Priyansh** + Marta)

DataSet: 

1. Count Table : Cryopreserved bone marrow stem/**progenitor CD34+** cells from 3 **healthy** donors. 10x dataset. 3 different samples
2. Count table: Patient 17: MDS and AML states from Guess et. al