---
title: "Introduction to single-cell technologies"
canonical: "https://help.biobam.com/space/BTP/3345973262/Introduction%20to%20single-cell%20technologies"
format: markdown
---
**Duration:** 60 min

**Lecture material:** 

- [Slides PDF](https://drive.google.com/file/d/13sJv6cVR176OCEPXAp4wvz2Pbj7x-aow/view?usp=sharing)


**Outline: **

- Introduction: what are single-cell technologies?
- Single-cell data generation:
  - Sample preparation methods: single-cell isolation
  - Library preparation and sequencing methods
    - 3'/5' end tagging methods: 10X Chromium
    - Full-length methods: Smart-seq2
    - Long-read sequencing methods
- 3’/5' end vs full-length data:
  - Transcript coverage
  - Sensitivity, sequencing depth, capture efficiency
  - Precision and accuracy for quantification
  - Cell throughput and power
  - Use-cases
- Multimodal single-cell data


**References:**

Paper references used throughout the preparation of this lecture are included in the corresponding slides.