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title: "RNA-Seq Data Analysis Workshop - Sevilla 2019"
canonical: "https://help.biobam.com/space/BTP/641171457/RNA-Seq%20Data%20Analysis%20Workshop%20-%20Sevilla%202019"
format: markdown
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Date : 27.3.2019 -29.3.2019 (3 days, 18 hours) Location : Estación Biológica de Doñ ana,  Avd. Américo Vespucio 26, Isla de la Cartuja, 41092 Sevilla, Spain Teachers : 3 Bioinformatics Experts from BioBam Bioinformatics S.L., Valencia, Spain Language:   Presentations in Spanish, Course Material in English Target Audience (24 max.):  Biologists with no or little experience in analyzing RNA-Seq data.  Scope : Complete bioinformatics workflow of a de-novo transcriptomics data analysis will we covered in a 3-day workshop with theoretical (~60%) as well as practical hands-on sessions (~40%). We will start with raw reads and discuss all steps until the biological interpretation of functional analysis results. The workshop design takes into consideration that we are treating with non-model organisms were no or little information exists in public databases. We will discuss up-to-date bioinformatics software solutions and provides hints about its execution including parameter setting, common pitfalls as well as input, output and system requirements. Day 1 Course Presentation Introduction to Sequencing Technologies Introduction to Bioinformatics Preprocessing and Quality Control of Raw Reads Hands-On Day 1    (intro slides as pdf ) Day 2 RNA-Seq De-novo Assembly Functional Annotation of Novel Sequence Data (Blast2GO Methodology) Hands-On Day 2 Day 3 Read Mapping and Expression Quantification   Statistical Analysis of Differential Gene/Isoform Expression Functional analysis via Enrichment Analysis (Fisher's and GSEA) Hands-On Day 3 Feedback Form and Free Trial Request Course Summary